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Recombinant DNA Technology
In this lecture ´´Recombinant DNA: Developments and Techniques´´ are explained. Section one is about ´´Recombinant DNA Technology´´. In the very first slide of this section recombinant DNA technology is introduced. In the next few slides restriction enzymes are focused. Educator also conveys information on artificial restriction enzymes. Lastly, interesting facts are presented.
Genetic Cloning and cDNA synthesis
Section two is about ‘’Genetic Cloning and cDNA synthesis’’. Educator illustrates the process of genetic cloning in detail. Then discussion continues on cDNA. Subsequently, cDNA synthesis is explained comprehensively.
Gel Electrophoresis and Southern Blotting
Section three is about ‘’Gel Electrophoresis and Southern Blotting’’. Educator talks about gel electrophoresis and tells that it was first introduced in 1930’s. Initially, discussion on history continues then the procedure of gel electrophoresis is explained. Educator also gives information about southern blotting. After that hybridization probes are pursued. Finally, the comprehensive explanation of procedure of southern blotting is presented.
Streptavidin-Biotin Binding and PCR
Section four is about ‘’Streptavidin-Biotin Binding and PCR’’. At first streptavidin-biotin binding and biotechnology is pursued. This is followed by the explanation of working principle of streptavidin-biotin. Likewise, applications of streptavidin-biotin are reported. Moreover, polymerase chain reaction is focused.
PCR Types and Applications
Section five is about ‘’PCR Types and Applications’’. Initially information is given on amplification and DNA copies. Then educator describes common types of PCR. Following this PCR efficiency is illustrated. Afterwards, applications of PCR are highlighted. Finally, use of PCR in cloning DNA fragments is discussed.