Recombinant DNA: Developments and Techniques

Microbiology

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Fmnypiditeoumpgliyws 180713 s0 arfeen zain recombinant dna developments and techniques intro
03:13
Recombinant DNA: Developments and Techniques
Go30acyisnegajn5go5p 180713 s1 arfeen zain recombinant dna technology
11:20
Recombinant DNA Technology
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08:46
Genetic Cloning and cDNA synthesis
Ndzscporeeorfaywrmrq 180713 s3 arfeen zain gel electrophoresis and southern blotting
11:40
Gel Electrophoresis and Southern Blotting
Abmewcqosbsalxbehkug 180713 s4 arfeen zain streptavidin biotin binding and pcr
10:40
Streptavidin-Biotin Binding and PCR
Wkuwssbvq0ixd7u0rlpo 180713 s5 arfeen zain pcr types and applications
10:08
PCR Types and Applications

Lecture´s Description

Recombinant DNA Technology

In this lecture ´´Recombinant DNA: Developments and Techniques´´ are explained. Section one is about ´´Recombinant DNA Technology´´. In the very first slide of this section recombinant DNA technology is introduced. In the next few slides restriction enzymes are focused. Educator also conveys information on artificial restriction enzymes. Lastly, interesting facts are presented.

Genetic Cloning and cDNA synthesis

Section two is about ‘’Genetic Cloning and cDNA synthesis’’. Educator illustrates the process of genetic cloning in detail. Then discussion continues on cDNA. Subsequently, cDNA synthesis is explained comprehensively.

Gel Electrophoresis and Southern Blotting

Section three is about ‘’Gel Electrophoresis and Southern Blotting’’. Educator talks about gel electrophoresis and tells that it was first introduced in 1930’s. Initially, discussion on history continues then the procedure of gel electrophoresis is explained. Educator also gives information about southern blotting. After that hybridization probes are pursued. Finally, the comprehensive explanation of procedure of southern blotting is presented.

Streptavidin-Biotin Binding and PCR

Section four is about ‘’Streptavidin-Biotin Binding and PCR’’. At first streptavidin-biotin binding and biotechnology is pursued. This is followed by the explanation of working principle of streptavidin-biotin. Likewise, applications of streptavidin-biotin are reported. Moreover, polymerase chain reaction is focused.

PCR Types and Applications

Section five is about ‘’PCR Types and Applications’’. Initially information is given on amplification and DNA copies. Then educator describes common types of PCR. Following this PCR efficiency is illustrated. Afterwards, applications of PCR are highlighted. Finally, use of PCR in cloning DNA fragments is discussed.

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