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Recombinant DNA and Plasmids
In this lecture ´´Recombinant DNA: Construction and Expression´´ is explained. Section one is about ‘‘Recombinant DNA and Plasmids’’. In the very first slide of this section, educator gives an overview of components of a cloning vector. After that light is shed on plasmids. This is followed by discussion of origin of replication. In the end selectable marker are highlighted.
Section two is about ‘’Cloning’’. Educator comprehensively introduces multicloning site or polylinker. Next focus is LacZ selection, a procedure to differentiate transformed and untransformed cells. Likewise, blue/white selection is discussed. Moving onward, recombinant plasmid construction and cloning comes under consideration. This is followed by recombinant plasmid cloning and selection.
Phage Vectors, Cosmids and Artificial Chromosomes
Section three is about ‘’Phage Vectors, Cosmids and Artificial Chromosomes’’. At first educator gives information about phage vectors. After that cosmids are highlighted. Moreover, detailed discussion continues on artificial chromosomes.
Section four is about ‘’Genomic Libraries’’. Initially, importance of genomic libraries is given. Then construction of genomic library is focused. This is followed by phenotypic rescue. Additionally, eukaryotic genomic library is introduced. Next, library construction is elucidated. Then library screening is illustrated. In the end of this section, educator shows how Introducing recombinant DNA into host cells is carried out.
Expression of Foreign Genes
Section five is about ‘’Expression of Foreign Genes’’. Earlier in this section, educator talks about heterologous genes. After that expression vectors are explained. Then process of protein purification is elucidated. Following this, His-Tagging is discussed. Subsequently, fluorescence labelling is reported.